© 2006 Society of Systematic Biologists
Utility of Nuclear Allele Networks for the Analysis of Closely Related Species in the Genus Carabus, Subgenus Ohomopterus
Department of Zoology, Graduate School of Science, Kyoto University Sakyo, Kyoto 606–8502, Japan E-mail:sota{at}terra.zool.kyoto-u.ac.jp
Edited by Marshal Hedin: Associate Editor
 |
Abstract |
|---|
Nuclear DNA sequence data for diploid organisms are potentially a rich source of phylogenetic information for disentangling the evolutionary relationships of closely related organisms, but present special phylogenetic problems owing to difficulties arising from heterozygosity and recombination. We analyzed allelic relationships for two nuclear gene regions (phosphoenolpyruvate carboxykinase and elongation factor-1
), along with a mitochondrial gene region (NADH dehydrogenase subunit 5), for an assemblage of closely related species of carabid beetles (Carabus subgenus Ohomopterus). We used a network approach to examine whether the nuclear gene sequences provide substantial phylogenetic information on species relationships and evolutionary history. The mitochondrial gene genealogy strongly contradicted the morphological species boundary as a result of introgression of heterospecific mitochondria. Two nuclear gene regions showed high allelic diversity within species, and this diversity was partially attributable to recombination between various alleles and high variability in the intron region. Shared nuclear alleles among species were rare and were considered to represent shared ancestral polymorphism. Despite the presence of recombination, nuclear allelic networks recovered species monophyly more often and presented genetic differentiation patterns (low to high) among species more clearly. Overall, nuclear gene networks provide clear evidence for separate biological species and information on the phylogenetic relationships among closely related carabid beetles.
Keywords: Gene genealogy; introgression; mitochondrial DNA; nuclear DNA; phylogeny; recombination; species relationship
Received June 18, 2005; Revised August 12, 2005; Accepted October 25, 2005
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Sota and N. Nagata Diversification in a fluctuating island setting: rapid radiation of Ohomopterus ground beetles in the Japanese Islands Phil Trans R Soc B, October 27, 2008; 363(1508): 3377 - 3390. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Morrison Increasing the Efficiency of Searches for the Maximum Likelihood Tree in a Phylogenetic Analysis of up to 150 Nucleotide Sequences Syst Biol, December 1, 2007; 56(6): 988 - 1010. [Abstract] [Full Text] [PDF]
|
|