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Systematic Biology Advance Access originally published online on July 6, 2009
Systematic Biology 2009 58(4):445-451; doi:10.1093/sysbio/syp033
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© Society of Systematic Biologists

Barcoding Bamboozled by Bacteria: Convergence to Metazoan Mitochondrial Primer Targets by Marine Microbes

Mark E. Siddall1,*, Frank M. Fontanella2, Sara C. Watson1, Sebastian Kvist3 and Christer Erséus4

1 Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, New York, USA
2 The Graduate Center, City University of New York, New York, New York, USA
3 Richard Gilder Graduate School, American Museum of Natural History, New York, New York, USA
4 Department of Zoology, University of Gothenburg, Göteborg, Sweden

* Correspondence to be sent to: Sackler Institute for Comparative Genomics, American Museum of Natural History, New York, New York, USA; E-mail: siddall@amnh.org.

Received September 28, 2008; Revised January 21, 2009; Accepted April 5, 2009
The first 150 words of the full text of this article appear below.

DNA barcoding has become a legitimate tool for assessment of global biodiversity patterns in a manner intended to allow diagnoses of known species to non-taxonomists. Relying on a standardized region (~ 650 nucleotides) of the cytochrome c oxidase subunit I mitochondrial gene (COI), a variety of taxon-based initiatives (www.barcoding.si.edu/major_projects.html), as well as large-scale locality-based barcoding projects (e.g., bscit.berkeley.edu/biocode/), are well underway. If DNA barcoding is to achieve its promise, these projects are essential as they will provide much-needed comparative baseline data for taxonomic groups, or for regional assemblages, upon which future identification efforts will rely. Thorough coverage of clades and guilds is essential to the success of DNA barcoding, but so too is highly accurate taxonomic identification of the source organisms. Although surreptitious amplification of nuclear mitochondrial pseudogenes (numts) may lead the unwary to overestimation of species diversity (Song et al. 2008), it may not . . . [Full Text of this Article]


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